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Tumor vaccine is a promising immunotherapy that involves incubation of tumor antigen-pulsed or genetically modified antigen-presenting cells (APC) with cytotoxic T lymphocytes (CTL). Our studies have revealed that there are two important types of co-stimulatory signals which are required for efficient priming and activation of T cells and regulation of the activity of CTL: one signaling pathway is initiated by contact of a B cell antigen receptor (BCR) on B cells with CD40 ligand on an APC, and the second signaling pathway is mediated by interaction of CD28 on T cells with the B7-family members on an APC. Signal via the BCR is necessary for efficient induction of co-stimulatory molecule expression on APC, and expression of B7-family members is an essential co-stimulatory signal for most types of antigen presenting cells (APC). Signal via the BCR also induces T cell receptor on T cells to remain in an active state, and depletes intr
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